Identification of Upper Respiratory Bacterial Pathogens With the Electronic Nose
電子鼻用于上呼吸道感染細菌病原體鑒定
Stephen Y. Lai MD, PhD Olivia F. Deffenderfer BS William Hanson MD Marguerite P. Phillips RN, BSN Erica R. Thaler MD
First published: 02 January 2009 https://doi.org/10.1097/-200206000-00007 Cited by: 47
Presented at the Eastern Section Meeting of the Triological Society, Philadelphia, PA, January 26, 2002.
Abstract
Objective:To use an electronic nose to identify common upper respiratory bacterial pathogens. Study Design: Controlled in vitro analysis.
目的：利用電子鼻對常見上呼吸道細菌病原進行鑒定。研究設計：體外對照分析。
Methods:Swabs of bacteria were obtained from in vitro samples. The specimens were vaporized and analyzed over the organic semiconductor-based electronicnose (Cyranose 320). Data from the 32-element sensor array were subjected to principal component analysis for depiction in two-dimensional space and difference in odorant patterns were assessed by calculating Mahalanobis distances.
方法：從體外標本中提取細菌拭子。樣品在有機半導體電子鼻(Cyranose320)上蒸發和分析。對32元傳感器陣列的數據進行主成分分析，在二維空間中進行描述，并通過計算馬哈拉諾比距離評估氣味模式的差異。
Results: The electronicnose was able to distinguish between control swabsand bacterial samples. Furthermore, calculation of the Mahalanobis distances among the various bacteria demonstrated distinct odorant classes (Mahalanobis distance 3). This demonstrates that the electronicnose could differentiate among various common bacterial pathogens of the upper respiratory tract, including Staphylococcus aureus, Streptococcus pneumoniae, Haemophilus influenza, and Pseudomonas aeruginosa. Conclusions: The electronic nose represents a novel method to identify potential upper respiratory infections and to discriminate among common upper respiratory bacterial pathogens. This technology could provide a rapid means to identify organisms causing upper respiratory infections.
結果：電子鼻能夠區分對照拭子和細菌樣本。此外，計算不同細菌之間的馬哈拉諾比斯距離顯示出不同的氣味類別(馬哈拉諾比斯距離3)。這表明，電子鼻可以區分上呼吸道常見的各種細菌病原體，包括*、肺炎鏈球菌、流感嗜血桿菌和銅綠假單胞菌。結論：電子鼻是一種新的鑒別上呼吸道感染的方法，是鑒別上呼吸道常見病原菌的一種新方法。這項技術可以提供一種快速的手段來鑒定引起上呼吸道感染的生物體。
Keywords: Electronic nose, bacteria, sinusitis.
