Current methods for detecting ethylene in plants
植物中的乙烯檢測(cè)方法
Simona M. Cristescu1,*, Julien Mandon1, Denis Arslanov1, Je´roˆme De Pessemier2, Christian Hermans2
and Frans J. M. Harren1
1Department of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135,
NL 6525 AJ Nijmegen, The Netherlands and 2Laboratory of Plant Physiology and Molecular Genetics, Universite´ Libre de
Bruxelles, Campus Plaine CP 242, Bd du Triomphe, 1050 Brussels, Belgium
* For correspondence. s.cristescu@science.ru.nl
Received: 29 August 2012 Revision requested: 1 October 2012 Accepted: 30 October 2012 Published electronically: 12 December 2012
Background
In view of ethylene's critical developmental and physiological roles the gaseous hormone remains an active research topic for plant biologists. Progress has been made to understand the ethylene biosynthesis pathway and the mechanisms of perception and action. Still numerous questions need to be answered and findings to be validated. Monitoring gas production will very often complete the picture of any ethylene research topic. Therefore the search for suitable ethylene measuring methods for various plant samples either in the field, greenhouses, laboratories or storage facilities is strongly motivated.
考慮到乙烯的關(guān)鍵發(fā)展和生理作用，氣態(tài)激素仍然是植物生物學(xué)家活躍的研究課題。在了解乙烯生物合成途徑以及感知和作用機(jī)理方面已取得進(jìn)展。仍然需要回答許多問題，并驗(yàn)證發(fā)現(xiàn)。監(jiān)測(cè)天然氣生產(chǎn)通常會(huì)完成任何乙烯研究主題的描述。因此，強(qiáng)烈地尋求在田間，溫室，實(shí)驗(yàn)室或存儲(chǔ)設(shè)施中為各種植物樣品尋找合適的乙烯測(cè)量方法
Scope
This review presents an update of the current methods for ethylene monitoring in plants. It focuses on the three most-used methods -gas chromatography detection, electrochemical sensing and optical detection – and compares them in terms of sensitivity, selectivity, time response and price. Guidelines are provided for proper selection and application of the described sensor methodologies and some specific applications are illustrated of laser-based detector for monitoring ethylene given off by Arabidopsis thaliana upon various nutritional treatments.
這篇綜述介紹了植物中乙烯監(jiān)測(cè)的方法。它著重介紹的方法-氣相色譜檢測(cè)，電化學(xué)傳感和光學(xué)檢測(cè)-并在靈敏度，選擇性，時(shí)間響應(yīng)和價(jià)格方面進(jìn)行比較。為正確選擇和應(yīng)用所描述的傳感器方法提供了指南，并舉例說明了基于激光的檢測(cè)器的一些特定應(yīng)用，該檢測(cè)器用于監(jiān)測(cè)擬南芥在各種營(yíng)養(yǎng)處理后釋放出的乙烯
Conclusions
Each method has its advantages and limitations. The choice for the suitable ethylene sensor needs careful consideration and is driven by the requirements for a specific application.
每種方法都有其優(yōu)點(diǎn)和局限性。合適的乙烯傳感器的選擇需要仔細(xì)考慮，并且受特定應(yīng)用要求
Keywords: Ethylene, Arabidopsis thaliana, gas sampling, gas chromatography, electrochemical sensing, laser-based detector
關(guān)鍵詞：乙烯、擬南芥、氣體采樣、氣相色譜、電化學(xué)傳感器、激光探測(cè)器
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