Medicinal cannabis: Principal cannabinoids concentration and theirstability evaluated by a high performance liquid chromatographycoupled to diode array and quadrupole time of flight massspectrometry method
藥用：用二極管陣列和四極飛行時間質譜法聯合高效液相色譜評價素的主要濃度和穩定性
Cinzia Cittia,b, Giuseppe Ciccarellaa,b, Daniela Braghirolic, Carlo Parentic,Maria Angela Vandellic, Giuseppe Cannazzab,c,∗
aDipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita`del Salento, Via per Monteroni,Lecce, Italy
bCNR NANOTEC, Campus Ecoteckne dell’Università del Salento, Via per Monteroni,Lecce, Italy
cDipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via Campi 103,Modena, Italy
Abstract
In the last few years, there has been a boost in the use of cannabis-based extracts for medicinal purposes,although their preparation procedure has not been standardized but rather decided by the individualpharmacists. The present work describes the development of a simple and rapid high performance liquidchromatography method with UV detection (HPLC-UV) for the qualitative and quantitative determinationof the principal cannabinoids (CBD-A, CBD, CBN, THC and THC-A) that could be applied to all cannabis-based medicinal extracts (CMEs) and easily performed by a pharmacist. In order to evaluate the identityand purity of the analytes, a high-resolution mass spectrometry (HPLC-ESI-QTOF) analysis was also car-ried out. Full method validation has been performed in terms of specificity, selectivity, linearity, recovery,dilution integrity and thermal stability. Moreover, the influence of the solvent (ethyl alcohol and oliveoil) was evaluated on cannabinoids degradation rate. An alternative extraction method has then beenproposed in order to preserve cannabis monoterpene component in final CMEs.
在過去幾年中，用于醫療目的的提取物的使用有所增加，盡管其制備程序尚未標準化，而是由個別藥劑師決定。本文介紹了一種簡便、快速、高效的紫外檢測液相色譜法(HPLC-UV)對素類化合物(CBD-a、CBD、CBN、CBN)進行定性和定量分析的方法，THC和THC-A)可應用于所有類藥物提取物(CME)，且易于由藥劑師執行。為了評價分析物的鑒別和純度，還進行了高分辨質譜(HPLC-ESI-QTOF)分析。在特異性、選擇性、線性、回收率、稀釋完整性和熱穩定性方面進行了全方法驗證?？疾炝巳軇?乙醇和橄欖油)對素降解率的影響。為了在終的CMEs中保存單萜成分，提出了另一種提取方法。
