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調制葉綠素熒光成像系統——IMAGING-PAM

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詳細信息 在線詢價

主要功能

  • 成像功能:對 Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等至少 17 種參數進行成像分析。測定調節性能量耗散 Y(NPQ),反映植物光保護能力,測定非調節性能量耗散Y(NO),反映植物光損傷程度。

  • 程序測量功能:可程序測量熒光誘導曲線、快速光曲線和暗弛豫,也可手動測量;在測量過程中能自動分析所有熒光參數的變化趨勢

  • AOI 功能:可在測量前或測量后任意選擇感興趣的區域(AOI),程序將自動對選擇的 AOI 的數據進行變化趨勢分析,并在報告文件中顯示相關 AOI 的數據。所有報告文件中顯示的數據都可導出到 EXCEL 文件中。

  • 成像異質性分析功能:對任意參數任意時間的成像,可在圖像上任意選取兩點,軟件自動對兩點間的數據進行橫向異質性分析,并可導出到 EXCEL 文件中。

  • 成像數據范圍分析功能:對任意參數任意時間的成像,可分析任意兩個熒光數值之間有多少個像素點,多少面積(cm2)。

  • 突變株篩選功能:可跟據成像結果快速篩選光合、產氫/油、抗逆(抗鹽、抗旱、抗病等)等突變株。

  • 微藻毒理研究功能:可同時測量 96 個微藻樣品(對照和處理組)的光合活性,軟件自動給出處理組樣品相對于對照組的光合抑制百分比。

  • 吸光系數測量功能:快速測量葉片的吸光系數。吸光系數測量光源: 16 個紅光(650 nm)和 16 個近紅外(780 nm)LED,用于測量植物葉片或藻類樣品 PAR 吸光系數。

 

測量參數

Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

 

應用領域

  • 光合作用研究:可以在*相同的條件下同時對大量樣品進行成像

  • 植物病理學:病斑部位(包括肉眼不可見時)成像以及病斑擴散的時空動力學

  • 植物脅迫生 理學:肉眼不可見脅迫損傷的早期檢測

  • 遺傳育種:出苗后大規??焖俸Y選高光合/抗旱/抗熱/抗凍/抗病等植株

  • 突變株篩選:快速篩選模式植物的光合突變株、抗逆突變株、產氫微藻突變株等

  • 微藻毒理學:不同毒物濃度多個重復的樣品一次測完,軟件自動計算抑制比率

  • 分子生物學:宏觀水平上檢測樣品的綠色熒光蛋白(GFP)熒光

  • 其它多種擴展研究

 

主要技術參數

MAXI 探頭(藍光版)

  • 熒光測量光源: 44 個藍色 LED,450 nm,測量光強度 0.5 μmol m-2 s-1,大光化光強度  1200 μmol m-2 s-1,飽和脈沖強度 2800 μmol m-2 s-1

  • 吸光系數測量光源:16個紅光(650 nm)和 16 個近紅外(780 nm)LED,用于測量樣品 PAR 吸光系數。

  • 成像面積:10×13 cm。

  • 光強異質性:測量區域光強異質性小于 ±7%。

  • 測量參數:Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

 

MAXI 探頭(紅光版)

  • 熒光測量光源: 44 個紅色 LED,620 nm,測量光強度 0.5 μmol m-2 s-1,大光化光強度 1200 μmol m-2 s-1,飽和脈沖強度 2800 μmol m-2 s-1。

  • 吸光系數測量光源:16 個紅光(650 nm)和 16 個近紅外(780 nm)LED,用于測量樣品 PAR 吸光系數。

  • 成像面積:10×13 cm。

  • 光強異質性:測量區域光強異質性小于 ±7%。

  • 測量參數:Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

 

MINI 探頭(藍光版,MINI 探頭成像方便野外使用)

  • 熒光測量光源:12 個藍色 LED,460 nm,測量光強度 0.5 μmol m-2 s-1,大光化光強度 2000 μmol m-2 s-1,飽和脈沖強度 6000 μmol m-2 s-1。

  • 吸光系數測量光源:10 個紅光(650 nm)和 10 個近紅外(780 nm)LED,用于測量樣品 PAR 吸光系數。

  • 信號檢測:1/3” 數碼相機 CCD,640×480 象素,采集速率 30 幀/秒,帶選擇性鎖相放大器。通過千兆以太網(GigE)與電腦數據通訊。

  • 成像面積:24×32 mm,6倍放大。

  • 測量參數:Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

 

MINI 探頭(紅光版,MINI 探頭成像方便野外使用)

  • 熒光測量光源:12 個紅色 LED,620 nm,測量光強度 0.5 μmol m-2 s-1,大光化光強度 2000 μmol m-2 s-1,飽和脈沖強度 6000 μmol m-2 s-1。

  • 吸光系數測量光源:10個紅光(650 nm)和 10 個近紅外(780 nm)LED,用于測量樣品 PAR 吸光系數。

  • 信號檢測:1/3” 數碼相機CCD,640×480 象素,采集速率 30 幀/秒,帶選擇性鎖相放大器。通過千兆以太網(GigE)與電腦數據通訊。

  • 成像面積:24×32 mm,6倍放大。

  • 測量參數:Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

 

選購指南

一、熒光成像 MAXI 版

系統組成:通用型主機,LED 供電單元, LED 光源,CCD,數據線,軟件等。

注意:高等植物或真核藻類測量時選擇藍光光源(*),藍藻測量請選擇紅光光源。

DSC_0917_CMYK-2.jpgI-PAM_MAXI-1.jpg
熒光成像 MAXI 版本

  

二、熒光成像 MINI 版

系統組成:通用型主機(下圖中未顯示),LED 光源(如下圖),CCD,數據線,軟件等。

注意:高等植物或真核藻類測量時選擇藍光光源(*),藍藻測量請選擇紅光光源。

DSC_1707-1.jpg
熒光成像 MINI 版

 

三、熒光成像 MICROSCOPY 版

系統組成:通用型主機,特制顯微鏡,CCD,數據線,軟件等。

DSC_2497_2-1.jpg
熒光成像 MICROSCOPY 版

  

seite2c2.jpgseite2b2.jpgseite4b.jpg
葡萄葉片 Y(NPQ) 成像葡萄葉片 PS/50 成像結果MINI 版本成像結果



zebrina04fo.jpgImage.PNGKinetics.PNG
顯微版本成像結果軟件成像界面慢速誘導動力學曲線

 

產地:德國 WALZ

 

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數據來源:光合作用文獻 Endnote 數據庫,更新至 2016 年 9 月,文獻數量超過 6000 篇

原始數據來源:Google Scholar


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