Detection of UV Induced Plant Stress by CO2/H2O Gas Analysis and Online Ethylene Measurement

通過在線乙烯分析儀和光合儀檢測紫外線誘導的植物脅迫
N. Domuratha, D. Brohm and F.-G. Schröder
Faculty for Agriculture / Landscape Management
University of Applied Sciences Dresden
01326 Dresden
Germany
Keywords:Solenostemon scularoides, injury-free measurement, photosynthesis rate, C2H4

Abstract
Photosynthesis and transpiration as well as ethylene emissions in the root zone were measured on Solenostemon scularioides hybrids (syn. Coleus blumei
Benth). It should be figured out whether these parameters are suitable to detect UV induced plant stress. The experimental plants were treated with artificial light from
HPS lamps with additional UV-A and UV-B light. It was not possible to make clear statements about the stress status of the plants on the basis of the photosynthesis and transpiration measurements. The ethylene values of unstressed plants were in a clear daily rhythm in accordance with other studies. It was not possible to reproduce the switching cycles of the UV lamps in the ethylene values. Watering, both with nutrient solution and deionised water, caused changes in the C2H4-budget. The HPS + UV-A variant responded with the strongest amplitude to nutrient solution. In the subsequent period mean values remained at 120% of the original ethylene levels for several hours.

在Solenostemon scularioides雜交種上測定根區的光合作用和蒸騰作用以及乙烯排放量(co.Coleus blumei)廣藿香)。應該弄清楚這些參數是否適合檢測UV誘導的植物脅迫。用來自HPS燈的人造光處理實驗植物，其具有額外的UV-A和UV-B光。在光合作用和蒸騰測量的基礎上，不可能對植物的脅迫狀態做出明確的陳述。根據其他研究，無應激植物的乙烯值具有明顯的日常節律。不可能在乙烯值中再現UV燈的開關循環。用營養液和去離子水澆水引起C 2 H 4的變化-預算。HPS + UV-A變體對營養液的響應幅度。在隨后的時期內，平均值保持在原始乙烯水平的120%，持續數小時。

The CIRAS-1 (PP Systems) with the leaf cuvette PLC B (settings: RS ratio 100, 200 ml min-1 flow rate, 380 ppm CO2) was used for the CO2/H2O gas exchange measurements. The values were taken from the three youngest unfolded leaves per experimental plant at each run. Measurements were made on the first and fifth day at the same daytime. The UV light was switched on already 30 minutes at the appropriate plots. The used portable devise ETH-1010 (Fluid Analytics) provides real-time measurements of ethylene in air at concentrations as low as 10 ppb. The measurement unit sampled about a 200 ml air within an interval of three minutes. A multiposition valve was pre-connected to the ETH-1010 to perform several measurements in a cycle. The multiposition valve switched four reading points. This led to measurement cycles of about 12 minutes. Three samples from the root area of experimental plants and one sample of greenhouse air (control point) were analysed at each cycle. Random tests of the accuracy were made by connecting a calibration gas to the control point.

使用CIRAS-1(PP Systems)和葉室PLC B(設置：RS比100，200 ml min-1流速，380 ppm CO2)進行CO2/H2O氣體交換測量。這些值取自每株實驗植物在每次運行時年輕的三片展開葉。在同一天的天和第五天進行測量。在適當的位置，紫外線燈已經打開30分鐘。使用的便攜式乙烯分析儀 ETH-1010(Fluid Analytics)提供了空氣中乙烯濃度低至10ppb的實時測量。測量單元在三分鐘的間隔內對大約200毫升空氣進行采樣。一個多位置的閥門被預先連接到ETH-1010上，在一個周期內進行多次測量。多位置閥切換了四個讀數點。大約12分鐘的測量周期。對試驗植物根區的3個樣品和溫室空氣(控制點)的1個樣品進行了每個周期的分析。通過將校準氣體連接到控制點，對精度進行隨機測試。